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Projects. Life and Earth Sciences

Study of EBV, HHV-6A and HHV-6B MICRO-RNAs in plasma isolated exosomes of patients with multiple sclerosis: correlation with activity and progression of disease

Lead Researcher: Roberto Álvarez Lafuente
Research Centre: Hospital Universitario Clínico San Carlos. Instituto de Investigación Sanitaria San Carlos (IdISSC). Madrid.

Abstract: 

Roberto Álvarez LafuenteA longitudinal two-year follow-up study will be conducted in a Spanish population composed of at least 100 relapsing-remitting multiple sclerosis (RRMS) patients to analyze the expression levels of EBV, HHV-6A and HHV-6B miRNAs present in isolated plasma exosomes by PCR Array and their possible relationship with: A) Disease progression, which will be measured clinically by the EDSS (Expanded Disability Status Scale ) variation between the baseline visit and visits at 12 and 24 months, and radiologically through the variation in the number of T2 lesions between the baseline visit and 12 and 24 months later; B) The activity of the disease, which will be measured clinically by the annualized rate of relapses, and radiologically through the presence of gadolinium-enhancing lesions, measured over the two-year follow-up; C) The evolution of the viral load of EBV, HHV-6A and HHV-6B measured by quantitative real time PCR; D) the evolution of IgG and IgM antibody titers against EBV and HHV-6 as measured by ELISA; E) expression levels of HLA-DR and HLA-DQ by quantitative RT-PCR; patients' HLA will have been previously typed by Luminex. This project could allow us to deepen in the possible mechanisms of action through which these viruses could be contributing to the etiopathogenesis of MS, and also to have new therapeutic targets based on the intercellular communication established by the virus infected cells with the cells of their environment.


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